Magnitude-dependent response of osteoblasts regulated by compressive stress.
Sci Rep
; 7: 44925, 2017 03 20.
Article
en En
| MEDLINE
| ID: mdl-28317941
ABSTRACT
The present study aimed to investigate the role of magnitude in adaptive response of osteoblasts exposed to compressive stress. Murine primary osteoblasts and MC3T3-E1 cells were exposed to compressive stress (0, 1, 2, 3, 4, and 5 g/cm2) in 3D culture. Cell viability was evaluated, and expression levels of Runx2, Alp, Ocn, Rankl, and Opg were examined. ALP activity in osteoblasts and TRAP activity in RAW264.7 cells co-cultured with MC3T3-E1 cells were assayed. Results showed that compressive stress within 5.0 g/cm2 did not influence cell viability. Both osteoblastic and osteoblast-regulated osteoclastic differentiation were enhanced at 2 g/cm2. An increase in stress above 2 g/cm2 did not enhance osteoblastic differentiation further but significantly inhibited osteoblast-regualted osteoclastic differentiation. This study suggested that compressive stress regulates osteoblastic and osteoclastic differentiation through osteoblasts in a magnitude-dependent manner.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Osteoblastos
/
Estrés Mecánico
Límite:
Animals
Idioma:
En
Año:
2017
Tipo del documento:
Article