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New real time and conventional RT-PCRs for updated molecular diagnosis of infectious bronchitis virus infection (IBV) in chickens in Egypt associated with frequent co-infections with avian influenza and Newcastle Disease viruses.
Naguib, Mahmoud M; El-Kady, Magdy F; Lüschow, Dörte; Hassan, Kareem E; Arafa, Abdel-Satar; El-Zanaty, Ali; Hassan, Mohamed K; Hafez, Hafez M; Grund, Christian; Harder, Timm C.
  • Naguib MM; The Federal Research Institute for Animal Health, Friedrich-Loeffler-Institut, Suedufer 10, Greifswald Insel-Riems 17493, Germany; National Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Dokki, P.O. Box 246, Giza 12618, Egypt.
  • El-Kady MF; Poultry Disease Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.
  • Lüschow D; Institute of Poultry Diseases, Freie Universität Berlin, 14163 Berlin, Germany.
  • Hassan KE; Poultry Disease Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.
  • Arafa AS; National Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Dokki, P.O. Box 246, Giza 12618, Egypt.
  • El-Zanaty A; National Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Dokki, P.O. Box 246, Giza 12618, Egypt.
  • Hassan MK; National Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Dokki, P.O. Box 246, Giza 12618, Egypt.
  • Hafez HM; Institute of Poultry Diseases, Freie Universität Berlin, 14163 Berlin, Germany.
  • Grund C; The Federal Research Institute for Animal Health, Friedrich-Loeffler-Institut, Suedufer 10, Greifswald Insel-Riems 17493, Germany.
  • Harder TC; The Federal Research Institute for Animal Health, Friedrich-Loeffler-Institut, Suedufer 10, Greifswald Insel-Riems 17493, Germany. Electronic address: timm.harder@fli.de.
J Virol Methods ; 245: 19-27, 2017 07.
Article en En | MEDLINE | ID: mdl-28336367
ABSTRACT
In Egypt, currently two geographically restricted genotypes of the infectious bronchitis coronavirus (IBV) are circulating with detrimental effects for poultry industry. A sensitive real-time RT-PCR assay targeting the IBV nucleocapsid gene (N) was developed to screen clinical samples for presence of IBV. Conventional RT-PCRs amplifying hypervariable regions (HVRs 1-2 and 3) of the IBV S1 gene were developed and amplificates used for nucleotide sequence-based typing of IBV field strains in Egyptian chickens directly from clinical samples.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Enfermedades de las Aves de Corral / Pollos / Infecciones por Coronavirus / Virus de la Bronquitis Infecciosa / Reacción en Cadena de la Polimerasa de Transcriptasa Inversa / Coinfección / Reacción en Cadena en Tiempo Real de la Polimerasa Tipo de estudio: Diagnostic_studies / Risk_factors_studies Límite: Animals País como asunto: Africa Idioma: En Año: 2017 Tipo del documento: Article
Texto completo
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Enfermedades de las Aves de Corral / Pollos / Infecciones por Coronavirus / Virus de la Bronquitis Infecciosa / Reacción en Cadena de la Polimerasa de Transcriptasa Inversa / Coinfección / Reacción en Cadena en Tiempo Real de la Polimerasa Tipo de estudio: Diagnostic_studies / Risk_factors_studies Límite: Animals País como asunto: Africa Idioma: En Año: 2017 Tipo del documento: Article