New real time and conventional RT-PCRs for updated molecular diagnosis of infectious bronchitis virus infection (IBV) in chickens in Egypt associated with frequent co-infections with avian influenza and Newcastle Disease viruses.
J Virol Methods
; 245: 19-27, 2017 07.
Article
en En
| MEDLINE
| ID: mdl-28336367
ABSTRACT
In Egypt, currently two geographically restricted genotypes of the infectious bronchitis coronavirus (IBV) are circulating with detrimental effects for poultry industry. A sensitive real-time RT-PCR assay targeting the IBV nucleocapsid gene (N) was developed to screen clinical samples for presence of IBV. Conventional RT-PCRs amplifying hypervariable regions (HVRs 1-2 and 3) of the IBV S1 gene were developed and amplificates used for nucleotide sequence-based typing of IBV field strains in Egyptian chickens directly from clinical samples.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Enfermedades de las Aves de Corral
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Pollos
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Infecciones por Coronavirus
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Virus de la Bronquitis Infecciosa
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Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
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Coinfección
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Reacción en Cadena en Tiempo Real de la Polimerasa
Tipo de estudio:
Diagnostic_studies
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Risk_factors_studies
Límite:
Animals
País como asunto:
Africa
Idioma:
En
Año:
2017
Tipo del documento:
Article