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Aging-related Atg5 defect impairs neutrophil extracellular traps formation.
Xu, Fengying; Zhang, Chengmi; Zou, Zui; Fan, Erica K Y; Chen, Linsong; Li, Yuehua; Billiar, Timothy R; Wilson, Mark A; Shi, Xueyin; Fan, Jie.
  • Xu F; Department of Anaesthesiology, Changzheng Hospital, Second Military Medical University, Shanghai, China.
  • Zhang C; Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
  • Zou Z; Department of Anaesthesiology, Shanghai Xinghua Hospital, Jiaotong University School of Medicine, Shanghai, China.
  • Fan EKY; Department of Anaesthesiology, Changzheng Hospital, Second Military Medical University, Shanghai, China.
  • Chen L; Department of Biological Sciences, University of Pittsburgh School of Arts and Science, Pittsburgh, PA, USA.
  • Li Y; Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
  • Billiar TR; Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.
  • Wilson MA; Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
  • Shi X; Research and Development, Veterans Affairs Pittsburgh Healthcare System, Pittsburgh, PA, USA.
  • Fan J; Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Immunology ; 151(4): 417-432, 2017 08.
Article en En | MEDLINE | ID: mdl-28375544
Formation of neutrophil extracellular traps (NETs) is an important function of the innate immune system against infections. It has been proven that aging dysregulates immunity and impairs neutrophil function. However, the influence of aging on the ability to produce NETs has yet to be fully addressed. In this study, we tested the hypothesis that a lower level of autophagy in neutrophils from aged mice was responsible for the decrease in NET formation. We demonstrated that a broad range of Toll-like receptor 2 (TLR2) ligands could efficiently induce reactive oxygen species (ROS) -dependent NET release in young mice, but not in aged ones. We further explored that the difference between young and aged mice in TLR2 ligand-induced NETosis is the result of an Atg5 defect and subsequent impaired autophagy. Furthermore, we found that lower autophagy capacity led to not only reduced NET formation, but also increased apoptosis. Our results suggest an important role of Atg5 and autophagy in maintaining the function of NETs formation in response to infection and in regulating neutrophil death. Targeting autophagy-promoted NETs may present a therapeutic strategy to improve infection defence in an aged population.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Autofagia / Envejecimiento / Trampas Extracelulares / Proteína 5 Relacionada con la Autofagia / Neutrófilos Límite: Animals Idioma: En Año: 2017 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Autofagia / Envejecimiento / Trampas Extracelulares / Proteína 5 Relacionada con la Autofagia / Neutrófilos Límite: Animals Idioma: En Año: 2017 Tipo del documento: Article