Gastrointestinal Colonization of Candida Albicans Increases Serum (1â3)-ß-D-Glucan, without Candidemia, and Worsens Cecal Ligation and Puncture Sepsis in Murine Model.
Shock
; 49(1): 62-70, 2018 Jan.
Article
en En
| MEDLINE
| ID: mdl-28498297
ABSTRACT
The role of intestinal Candida albicans in bacterial sepsis, in the absence of candidemia, was investigated in murine models. Live C albicans or normal saline solution (NSS) was administered orally once, followed by 5 days of daily oral antibiotic-mixtures (ATB). Cecal ligation and puncture (CLP) was then performed to induce sepsis.Fecal Candida was detected by culture only in models with Candida administration. Oral Candida administration with/without ATB enhanced gut-pathogenic bacteria as determined by microbiome analysis. Despite negative candidemia, serum (1â3)-ß-D-glucan (BG) was higher in CLP with Candida preconditioning models than in CLP-controls (NSS-preconditioning) at 6 and/or 18âh post-CLP. Blood bacterial burdens were not increased with Candida administration.Additionally, CLP with high-dose Candida (10 colony forming units) induced higher levels of fecal Candida, serum BG, serum IL-6, and mortality than the lowest dose (100 colony forming units). Interestingly, fluconazole attenuated fecal Candida and improved survival in mice with live-Candida administration, but not in the CLP-controls. Heat-killed Candida preparations or their supernatants reduced bone marrow-derived macrophage killing activity in vitro but enhanced cytokine production.In conclusion, intestinal abundance of fungi and/or fungal-molecules was associated with increased bacterial sepsis severity, perhaps through cytokine storm induction and/or decreased macrophage killing activity. These observations suggest that further investigation of the potential role of intestinal fungal burdens in sepsis is warranted.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Candida albicans
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Sepsis
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Tracto Gastrointestinal
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Candidemia
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Glucanos
Límite:
Animals
Idioma:
En
Año:
2018
Tipo del documento:
Article