A simple and rapid method to obtain substitution mutations in Escherichia coli: isolation of a dam deletion/insertion mutation.
Gene
; 73(2): 531-5, 1988 Dec 20.
Article
en En
| MEDLINE
| ID: mdl-2854098
ABSTRACT
We describe the isolation of a strain of Escherichia coli bearing a deletion/insertion (i.e., a substitution mutation) in the dam gene (dam-16). The mutagenesis protocol used should be applicable to any cloned non-essential gene of E. coli. The substitution mutation confers resistance to kanamycin and can easily be transferred to other strains by standard genetic techniques. The amount of Dam methyltransferase (MTase) in dam-16 strains as determined either in vitro or in vivo is below the level of detection. We conclude that the Dam MTase is not required for viability of E. coli.
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Banco de datos:
MEDLINE
Asunto principal:
Elementos Transponibles de ADN
/
Metiltransferasa de ADN de Sitio Específico (Adenina Especifica)
/
Deleción Cromosómica
/
Escherichia coli
/
Genes
/
Genes Bacterianos
/
Mutación
Idioma:
En
Año:
1988
Tipo del documento:
Article