Mouse model of hematogenous implant-related Staphylococcus aureus biofilm infection reveals therapeutic targets.
Proc Natl Acad Sci U S A
; 114(26): E5094-E5102, 2017 06 27.
Article
en En
| MEDLINE
| ID: mdl-28607050
ABSTRACT
Infection is a major complication of implantable medical devices, which provide a scaffold for biofilm formation, thereby reducing susceptibility to antibiotics and complicating treatment. Hematogenous implant-related infections following bacteremia are particularly problematic because they can occur at any time in a previously stable implant. Herein, we developed a model of hematogenous infection in which an orthopedic titanium implant was surgically placed in the legs of mice followed 3 wk later by an i.v. exposure to Staphylococcus aureus This procedure resulted in a marked propensity for a hematogenous implant-related infection comprised of septic arthritis, osteomyelitis, and biofilm formation on the implants in the surgical legs compared with sham-operated surgical legs without implant placement and with contralateral nonoperated normal legs. Neutralizing human monoclonal antibodies against α-toxin (AT) and clumping factor A (ClfA), especially in combination, inhibited biofilm formation in vitro and the hematogenous implant-related infection in vivo. Our findings suggest that AT and ClfA are pathogenic factors that could be therapeutically targeted against Saureus hematogenous implant-related infections.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Osteomielitis
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Infecciones Estafilocócicas
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Staphylococcus aureus
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Artritis Infecciosa
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Biopelículas
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Implantes Experimentales
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Anticuerpos Neutralizantes
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Anticuerpos Antibacterianos
Tipo de estudio:
Etiology_studies
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Prognostic_studies
Límite:
Animals
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Humans
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Male
Idioma:
En
Año:
2017
Tipo del documento:
Article