TSPAN7, effector of actin nucleation required for dendritic cell-mediated transfer of HIV-1 to T cells.
Biochem Soc Trans
; 45(3): 703-708, 2017 06 15.
Article
en En
| MEDLINE
| ID: mdl-28620031
ABSTRACT
Dendritic cells (DCs) have essential roles in early detection of pathogens and activation of both innate and adaptive immune responses. Whereas human DCs are resistant to productive HIV-1 replication, they have a unique ability to take up virus and transmit it efficiently to T lymphocytes. By doing that, HIV-1 may evade, at least in part, the first line of defense of the immune system, exploiting DCs instead to facilitate rapid infection of a large pool of immune cells. While performing an shRNA screen in human primary monocyte-derived DCs, to gain insights into this cell biological process, we discovered the role played by tetraspanin-7 (TSPAN7). This member of the tetraspanin family appears to be a positive regulator of actin nucleation and stabilization, through the ARP2/3 complex. By doing so, TSPAN7 limits HIV-1 endocytosis and maintains viral particles on actin-rich dendrites for an efficient transfer toward T lymphocytes. While studying the function of TSPAN7 in the control of actin nucleation, we also discovered the existence in DCs of two opposing forces at the plasma membrane actin nucleation, a protrusive force which seems to counterbalance actomyosin contraction.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Células Dendríticas
/
Linfocitos T
/
Actinas
/
VIH-1
/
Tetraspaninas
/
Proteínas del Tejido Nervioso
Tipo de estudio:
Screening_studies
Límite:
Humans
Idioma:
En
Año:
2017
Tipo del documento:
Article