Stabilization vs. destabilization of G-quadruplex superstructures: the role of the porphyrin derivative having spermine arms.
Phys Chem Chem Phys
; 19(26): 17404-17410, 2017 Jul 14.
Article
en En
| MEDLINE
| ID: mdl-28650039
ABSTRACT
The interaction of the porphyrin derivative H2TCPPSpm4, having spermine pendants in the four meso positions, with the G-quadruplex (GQ) structure formed by the DNA aptamer TGGGAG has been investigated by means of UV, electronic circular dichroism and PAGE studies. The results reported here demonstrate that the porphyrin derivative is capable of stabilizing or destabilizing the higher-ordered structures of parallel GQs, depending on the method used to reach their relative stoichiometry (titration vs. single addition). Noteworthily, when two equivalents of H2TCPPSpm4 were mixed directly with one equivalent of the (TGGGAG)4 GQ to reach a 2 1 H2TCPPSpm4 GQ ratio T1/2 higher than 80 °C was also observed confirming the presence of higher-ordered GQ structures.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Porfirinas
/
Espermina
/
G-Cuádruplex
Idioma:
En
Año:
2017
Tipo del documento:
Article