High-Efficiency Transfection of Glioblastoma Cells and a Simple Spheroid Migration Assay.

Hagemann, Carsten; Amend, Diana; Kessler, Almuth F; Linsenmann, Thomas; Ernestus, Ralf-Ingo; Löhr, Mario

Hagemann, Carsten; Amend, Diana; Kessler, Almuth F; Linsenmann, Thomas; Ernestus, Ralf-Ingo; Löhr, Mario.

Hagemann C; Tumorbiology Laboratory, Department of Neurosurgery, University of Würzburg, Josef-Schneider-Str. 11, 97080, Würzburg, Germany. hagemann_c@ukw.de.
Amend D; Tumorbiology Laboratory, Department of Neurosurgery, University of Würzburg, Josef-Schneider-Str. 11, 97080, Würzburg, Germany.
Kessler AF; Tumorbiology Laboratory, Department of Neurosurgery, University of Würzburg, Josef-Schneider-Str. 11, 97080, Würzburg, Germany.
Linsenmann T; Tumorbiology Laboratory, Department of Neurosurgery, University of Würzburg, Josef-Schneider-Str. 11, 97080, Würzburg, Germany.
Ernestus RI; Tumorbiology Laboratory, Department of Neurosurgery, University of Würzburg, Josef-Schneider-Str. 11, 97080, Würzburg, Germany.
Löhr M; Tumorbiology Laboratory, Department of Neurosurgery, University of Würzburg, Josef-Schneider-Str. 11, 97080, Würzburg, Germany.

Methods Mol Biol ; 1622: 63-79, 2017.

Article en En | MEDLINE | ID: mdl-28674801

Asunto(s)

Movimiento Celular/genética; Glioblastoma/genética; Glioblastoma/patología; Transfección; Línea Celular Tumoral; Ensayos de Migración Celular; Supervivencia Celular/genética; Expresión Génica; Técnicas de Inactivación de Genes; Humanos; Esferoides Celulares

Palabras clave

Glioblastoma multiforme; Glioma; Migration assay; Neurosphere; Nucleofection; Overexpression; Spheroid; Transfection; Western blotting; siRNA

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Transfección / Movimiento Celular / Glioblastoma Límite: Humans Idioma: En Año: 2017 Tipo del documento: Article

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