Surface functionalisation of nanodiamonds for human neural stem cell adhesion and proliferation.
Sci Rep
; 7(1): 7307, 2017 08 04.
Article
en En
| MEDLINE
| ID: mdl-28779095
ABSTRACT
Biological systems interact with nanostructured materials on a sub-cellular level. These interactions may govern cell behaviour and the precise control of a nanomaterial's structure and surface chemistry allow for a high degree of tunability to be achieved. Cells are surrounded by an extra-cellular matrix with nano-topographical properties. Diamond based materials, and specifically nanostructured diamond has attracted much attention due to its extreme electrical and mechanical properties, chemical inertness and biocompatibility. Here the interaction of nanodiamond monolayers with human Neural Stem Cells (hNSCs) has been investigated. The effect of altering surface functionalisation of nanodiamonds on hNSC adhesion and proliferation has shown that confluent cellular attachment occurs on oxygen terminated nanodiamonds (O-NDs), but not on hydrogen terminated nanodiamonds (H-NDs). Analysis of H and O-NDs by Atomic Force Microscopy, contact angle measurements and protein adsorption suggests that differences in topography, wettability, surface charge and protein adsorption of these surfaces may underlie the difference in cellular adhesion of hNSCs reported here.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Adhesión Celular
/
Células-Madre Neurales
/
Nanodiamantes
Límite:
Humans
Idioma:
En
Año:
2017
Tipo del documento:
Article