Hydroxylation of a conserved tRNA modification establishes non-universal genetic code in echinoderm mitochondria.
Nat Struct Mol Biol
; 24(9): 778-782, 2017 Sep.
Article
en En
| MEDLINE
| ID: mdl-28783151
ABSTRACT
The genetic code is not frozen but still evolving, which can result in the acquisition of 'dialectal' codons that deviate from the universal genetic code. RNA modifications in the anticodon region of tRNAs play a critical role in establishing such non-universal genetic codes. In echinoderm mitochondria, the AAA codon specifies asparagine instead of lysine. By analyzing mitochondrial (mt-) tRNALys isolated from the sea urchin (Mesocentrotus nudus), we discovered a novel modified nucleoside, hydroxy-N6-threonylcarbamoyladenosine (ht6A), 3' adjacent to the anticodon (position 37). Biochemical analysis revealed that ht6A37 has the ability to prevent mt-tRNALys from misreading AAA as lysine, thereby indicating that hydroxylation of N6-threonylcarbamoyladenosine (t6A) contributes to the establishment of the non-universal genetic code in echinoderm mitochondria.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Erizos de Mar
/
ARN de Transferencia de Lisina
/
Procesamiento Postranscripcional del ARN
/
Código Genético
/
Mitocondrias
Límite:
Animals
Idioma:
En
Año:
2017
Tipo del documento:
Article