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A microfluidics-based in situ chemotaxis assay to study the behaviour of aquatic microbial communities.
Lambert, Bennett S; Raina, Jean-Baptiste; Fernandez, Vicente I; Rinke, Christian; Siboni, Nachshon; Rubino, Francesco; Hugenholtz, Philip; Tyson, Gene W; Seymour, Justin R; Stocker, Roman.
  • Lambert BS; Department of Civil and Environmental Engineering, Ralph M. Parsons Laboratory, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
  • Raina JB; Department of Civil, Environmental and Geomatic Engineering, Institute for Environmental Engineering, ETH Zurich, 8093, Zurich, Switzerland.
  • Fernandez VI; Department of Applied Ocean Physics and Engineering, Woods Hole Oceanographic Institution, Woods Hole, MA, 02543, USA.
  • Rinke C; Climate Change Cluster, University of Technology Sydney, Ultimo, 2007, NSW, Australia.
  • Siboni N; Department of Civil and Environmental Engineering, Ralph M. Parsons Laboratory, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
  • Rubino F; Department of Civil, Environmental and Geomatic Engineering, Institute for Environmental Engineering, ETH Zurich, 8093, Zurich, Switzerland.
  • Hugenholtz P; Australian Centre for Ecogenomics, School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, 4072, QLD, Australia.
  • Tyson GW; Climate Change Cluster, University of Technology Sydney, Ultimo, 2007, NSW, Australia.
  • Seymour JR; Australian Centre for Ecogenomics, School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, 4072, QLD, Australia.
  • Stocker R; Australian Centre for Ecogenomics, School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, 4072, QLD, Australia.
Nat Microbiol ; 2(10): 1344-1349, 2017 Oct.
Article en En | MEDLINE | ID: mdl-28848238
ABSTRACT
Microbial interactions influence the productivity and biogeochemistry of the ocean, yet they occur in miniscule volumes that cannot be sampled by traditional oceanographic techniques. To investigate the behaviours of marine microorganisms at spatially relevant scales, we engineered an in situ chemotaxis assay (ISCA) based on microfluidic technology. Here, we describe the fabrication, testing and first field results of the ISCA, demonstrating its value in accessing the microbial behaviours that shape marine ecosystems.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Agua de Mar / Microbiología del Agua / Quimiotaxis / Fenómenos Fisiológicos Bacterianos / Microfluídica Idioma: En Año: 2017 Tipo del documento: Article
Texto completo
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XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Agua de Mar / Microbiología del Agua / Quimiotaxis / Fenómenos Fisiológicos Bacterianos / Microfluídica Idioma: En Año: 2017 Tipo del documento: Article