Efficient differentiation of Corynebacterium striatum, Corynebacterium amycolatum and Corynebacterium xerosis clinical isolates by multiplex PCR using novel species-specific primers.
J Microbiol Methods
; 142: 33-35, 2017 11.
Article
en En
| MEDLINE
| ID: mdl-28888870
ABSTRACT
A multiplex-PCR (mPCR) assay was designed with species-specific primers which generate amplicons of 226bp, 434bp and 106bp for differentiating the species C. striatum, C. amycolatum, and C. xerosis, respectively. mPCR results were 100% in agreement with identifications achieved by 16S rRNA and rpoB gene sequencing and by VITEK-MS.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Corynebacterium
/
Infecciones por Corynebacterium
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Tipificación Molecular
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Reacción en Cadena de la Polimerasa Multiplex
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Año:
2017
Tipo del documento:
Article