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Efficient differentiation of Corynebacterium striatum, Corynebacterium amycolatum and Corynebacterium xerosis clinical isolates by multiplex PCR using novel species-specific primers.
Santos, Carolina S; Ramos, Juliana N; Vieira, Veronica V; Pinheiro, Carina S; Meyer, Roberto; Alcantara-Neves, Neuza M; Ramos, Rommel T; Silva, Artur; Hirata, Raphael; Felicori, Liza; de Alegría Puig, Carlos Ruiz; Navas, Jesús; Azevedo, Vasco; Mattos-Guaraldi, Ana L; Pacheco, Luis G C.
  • Santos CS; Institute of Health Sciences, Federal University of Bahia (UFBA), Salvador, BA, Brazil.
  • Ramos JN; National Institute for Quality Control in Health (INCQS), Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, RJ, Brazil.
  • Vieira VV; Oswaldo Cruz Institute (IOC), Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, RJ, Brazil.
  • Pinheiro CS; Institute of Health Sciences, Federal University of Bahia (UFBA), Salvador, BA, Brazil.
  • Meyer R; Institute of Health Sciences, Federal University of Bahia (UFBA), Salvador, BA, Brazil.
  • Alcantara-Neves NM; Institute of Health Sciences, Federal University of Bahia (UFBA), Salvador, BA, Brazil.
  • Ramos RT; Institute of Biological Sciences, Federal University of Pará (UFPA), Belém, PA, Brazil.
  • Silva A; Institute of Biological Sciences, Federal University of Pará (UFPA), Belém, PA, Brazil.
  • Hirata R; Faculty of Medical Sciences, Rio de Janeiro State University (UERJ), Rio de Janeiro, RJ, Brazil.
  • Felicori L; Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte, MG, Brazil.
  • de Alegría Puig CR; Departamento de Biología Molecular, Universidad de Cantabria, Servicio de Microbiología, Hospital Universitario Marqués de Valdecilla, Santander, Spain.
  • Navas J; Departamento de Biología Molecular, Universidad de Cantabria, Servicio de Microbiología, Hospital Universitario Marqués de Valdecilla, Santander, Spain.
  • Azevedo V; Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte, MG, Brazil.
  • Mattos-Guaraldi AL; Faculty of Medical Sciences, Rio de Janeiro State University (UERJ), Rio de Janeiro, RJ, Brazil.
  • Pacheco LGC; Institute of Health Sciences, Federal University of Bahia (UFBA), Salvador, BA, Brazil. Electronic address: luis.pacheco@ufba.br.
J Microbiol Methods ; 142: 33-35, 2017 11.
Article en En | MEDLINE | ID: mdl-28888870
ABSTRACT
A multiplex-PCR (mPCR) assay was designed with species-specific primers which generate amplicons of 226bp, 434bp and 106bp for differentiating the species C. striatum, C. amycolatum, and C. xerosis, respectively. mPCR results were 100% in agreement with identifications achieved by 16S rRNA and rpoB gene sequencing and by VITEK-MS.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Corynebacterium / Infecciones por Corynebacterium / Tipificación Molecular / Reacción en Cadena de la Polimerasa Multiplex Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Año: 2017 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Corynebacterium / Infecciones por Corynebacterium / Tipificación Molecular / Reacción en Cadena de la Polimerasa Multiplex Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Año: 2017 Tipo del documento: Article