Removal and killing of multispecies endodontic biofilms by N-acetylcysteine.
Braz J Microbiol
; 49(1): 184-188, 2018.
Article
en En
| MEDLINE
| ID: mdl-28916389
ABSTRACT
Removal of bacterial biofilm from the root canal system is essential for the management of endodontic disease. Here we evaluated the antibacterial effect of N-acetylcysteine (NAC), a potent antioxidant and mucolytic agent, against mature multispecies endodontic biofilms consisting of Actinomyces naeslundii, Lactobacillus salivarius, Streptococcus mutans and Enterococcus faecalis on sterile human dentin blocks. The biofilms were exposed to NAC (25, 50 and 100mg/mL), saturated calcium hydroxide or 2% chlorhexidine solution for 7 days, then examined by scanning electron microscopy. The biofilm viability was measured by viable cell counts and ATP-bioluminescence assay. NAC showed greater efficacy in biofilm cell removal and killing than the other root canal medicaments. Furthermore, 100mg/mL NAC disrupted the mature multispecies endodontic biofilms completely. These results demonstrate the potential use of NAC in root canal treatment.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Acetilcisteína
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Streptococcus mutans
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Actinomyces
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Enterococcus faecalis
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Biopelículas
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Enfermedades de la Pulpa Dental
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Ligilactobacillus salivarius
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Antibacterianos
Límite:
Humans
Idioma:
En
Año:
2018
Tipo del documento:
Article