Unraveling the expression of genes involved in the biosynthesis pathway of cardiolipin and phosphatidylethanolamine in Salmonella Hadar grown under static magnetic field 200 mT.

ABSTRACT

We aimed in this work to evaluate the effect of static magnetic field 200 mT (SMF) on the expression of genes involved in the biosynthetic pathway of cardiolipin g3pd, plsB, cdsA, pgsA, pgpA, cls and phosphatidylethanolamine pssA and psd in Salmonella enterica subsp enterica serovar Hadar. Bacteria were exposed to a SMF during 3, 6 and 9 h. RNA extraction was followed by Reverse Transcriptase Polymerase Chain Reaction RT-PCR. The relative quantification of mRNA expression levels using 16S rRNA doesn't change during the time exposure. RT-PCR was done for two exposure experiments. The gene expression using RT-PCR present no significant difference in case of plsB, cdsA, pgpA, pgsA and psd genes during the different exposure times. However, a significant increase was observed in the expression of g3pd and pssA genes after 6 h and for cls gene after 3 h of exposure, but any variation was notified after 9 h of exposure. So we can conclude from this study that cls, g3pd and pssA genes are required in the adaptation of Salmonella Hadar to SMF.