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CRISPR/Cas9-mediated gene knockout is insensitive to target copy number but is dependent on guide RNA potency and Cas9/sgRNA threshold expression level.
Yuen, Garmen; Khan, Fehad J; Gao, Shaojian; Stommel, Jayne M; Batchelor, Eric; Wu, Xiaolin; Luo, Ji.
  • Yuen G; Laboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA.
  • Khan FJ; Laboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA.
  • Gao S; Undergraduate Scholarship Program, National Institutes of Health, Bethesda, MD, USA.
  • Stommel JM; Thoracic and Gastrointestinal Oncology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA.
  • Batchelor E; Radiation Oncology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA.
  • Wu X; Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA.
  • Luo J; Cancer Research Technology Program, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, MD, USA.
Nucleic Acids Res ; 45(20): 12039-12053, 2017 Nov 16.
Article en En | MEDLINE | ID: mdl-29036671
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Dosificación de Gen / Proteínas Fluorescentes Verdes / Técnicas de Inactivación de Genes / Sistemas CRISPR-Cas Límite: Humans Idioma: En Año: 2017 Tipo del documento: Article
Texto completo
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Dosificación de Gen / Proteínas Fluorescentes Verdes / Técnicas de Inactivación de Genes / Sistemas CRISPR-Cas Límite: Humans Idioma: En Año: 2017 Tipo del documento: Article