A uniform method for the simultaneous blood group phenotyping of Fya , Fyb , Jka , Jkb , S, sÌ
, P1, k applying lateral-flow technique.
Vox Sang
; 113(2): 177-184, 2018 Feb.
Article
en En
| MEDLINE
| ID: mdl-29044588
ABSTRACT
BACKGROUND AND OBJECTIVES:
A lateral flow assay for simultaneous blood group typing of ABO, RhD, C, E, c, e, Cw and K with stable end-point and without centrifugation is in routine use since several years (MDmulticard® ). The typing of extended phenotype parameters belonging to the Duffy, Kidd, MNSs blood group systems and others, however, has not yet been demonstrated for this technique. Reliable detection of Fyx , a weak Fyb phenotype with a pronounced quantitative reduction of the number of Fyb antigens on the erythrocyte surface, remains a weakness of current serological blood grouping techniques. MATERIAL ANDMETHODS:
The performance characteristics of the following reagents were evaluated in donor and patient samples in lateral flow technology (MDmulticard® ) Anti-Fya , -Fyb , -Jka , -Jkb , -S, -sÌ , -P1 and -k. The sensitivity to detect Fyx was in addition evaluated with Fyx positive samples, which had been preselected by MALDI-TOF MS-based genotyping.RESULTS:
All results obtained with the MDmulticard® were in full accordance with those of the CE-certified reference products for all the eight reagent formulations used Anti-Fya , -Fyb , -Jka , -Jkb , -S, -sÌ , -P1 and -k. Also, all Fyx phenotypes of the selected population of 93 positive samples, originally identified by MALDI-TOF MS-based genotyping, were reliably detected by the lateral flow assay.CONCLUSION:
Extended phenotype blood group parameters, including the serologically challenging Fyx phenotype, can be determined simultaneously, rapidly and accurately using the lateral flow (MDmulticard® ) technology, even in cases when IgG class antibodies are the only source of diagnostic antibodies.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Fenotipo
/
Tipificación y Pruebas Cruzadas Sanguíneas
/
Sistema del Grupo Sanguíneo Duffy
/
Sistema del Grupo Sanguíneo MNSs
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Humans
Idioma:
En
Año:
2018
Tipo del documento:
Article