The effects of supplemental vitamin E on hematological parameters in a rat model of ovarian hormone deficiency.
Menopause
; 25(3): 336-342, 2018 03.
Article
en En
| MEDLINE
| ID: mdl-29088013
ABSTRACT
OBJECTIVE:
Menopause is associated with adverse changes in hematological parameters. Although the antioxidative and anti-inflammatory properties of vitamin E have been previously demonstrated, the effects of vitamin E on hematopoietic parameters are not well-documented. This study investigated the effects of supplemental vitamin E on hematological parameters in a rat model of ovarian hormone deficiency.METHODS:
Twelve-month-old female Sprague-Dawley rats were either sham-operated (Sham) or ovariectomized (Ovx). Animals were randomly divided among five treatment groups (nâ=â12/group) as follows Sham; Ovx; Ovxâ+â300, Ovxâ+â525, or Ovxâ+â750âmg/kg diet of vitamin E for 100 days.RESULTS:
Compared with Sham, ovariectomy increased leukocyte subpopulation counts including lymphocytes (2.01â×â10/mm; 95% confidence interval [CI] 0.11, 4.03; Pâ=â0.03), monocytes (0.35â×â10/mm; 95% CI 0.60, 0.11; Pâ=â0.01), neutrophils (0.72â×â10/mm; 95% CI 0.26, 1.19; Pâ=â0.01), eosinophils (0.07â×â10/mm; 95% CI 0.12, 0.30; Pâ=â0.00), and basophils (0.13â×â10/mm; 95% CI 0.04, 0.21; Pâ=â0.02). Medium dose (MD) (-0.26â×â10/mm; 95% CI -0.47, -0.05; Pâ=â0.007) and high dose (HD) (-0.22â×â10/mm; 95% CI -0.43, -0.01; Pâ=â0.037) supplemental vitamin E attenuated Ovx-induced increases in monocyte counts. Low dose (LD) (-0.55â×â10/mm; 95% CI -0.95, -0.15; Pâ=â0.003), MD (-0.61â×â10/mm; Pâ=â0.001), and HD (-0.54â×â10/mm; 95% CI -0.95, -0.14; Pâ=â0.004) supplemental vitamin E attenuated Ovx-induced increases in neutrophil counts. LD (-0.05â×â10/mm; 95% CI -0.08, -0.11; Pâ=â0.006), MD (-0.05â×â10/mm; 95% CI -0.08, -0.11; Pâ=â0.005), and HD (-0.05â×â10/mm; 95% CI -0.09, -0.01; Pâ=â0.004) supplemental vitamin E also attenuated the Ovx-induced increase in eosinophil counts. Only LD (-0.09â×â10/mm; 95% CI -0.17, -0.02; Pâ=â0.009) supplemental vitamin E attenuated the Ovx-induced increase in basophil counts. The remaining hematological parameters assessed were not significantly affected by ovariectomy or supplemental vitamin E.CONCLUSION:
These findings suggest that vitamin E in the form of α-tocopherol acetate may provide protection against ovarian hormone deficiency-associated adverse changes in hematological parameters.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Vitamina E
/
Insuficiencia Ovárica Primaria
/
Leucocitos
/
Antioxidantes
Tipo de estudio:
Clinical_trials
/
Prognostic_studies
Límite:
Animals
/
Female
/
Humans
Idioma:
En
Año:
2018
Tipo del documento:
Article