Biological and biochemical characterization of M2B cells: Classical BSE prion is conserved in transgenic mice overexpressing bovine prion protein gene.
Prion
; 11(6): 405-414, 2017 11 02.
Article
en En
| MEDLINE
| ID: mdl-29098930
ABSTRACT
M2B cells with persistent classical bovine spongiform encephalopathy (C-BSE) have been established previously. In this study, we performed strain characterization of the M2B cell line in bovine PrPC overexpressing mice (Tg 1896). Mice intracranially inoculated with M2B cells and C-BSE survived for 451 ± 7 and 465 ± 31 d post inoculation, respectively. Although biochemical properties, including deglycosylation and conformational stability, differed between M2B cells and C-BSE, inoculation with M2B cell lysate and C-BSE resulted in comparable phenotypes. Comparable vacuolation scores and PrPSc depositions were observed in the brain of Tg 1896 inoculated with both M2B cell lysate and C-BSE. Our results show that biochemical and biological characteristics of M2B cells and C-BSE are classifiable in the same strain.
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Texto completo:
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Banco de datos:
MEDLINE
Asunto principal:
Bioensayo
/
Encefalopatía Espongiforme Bovina
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Proteínas Priónicas
Límite:
Animals
Idioma:
En
Año:
2017
Tipo del documento:
Article