The intron landscape of the mtDNA cytb gene among the Ascomycota: introns and intron-encoded open reading frames.
Mitochondrial DNA A DNA Mapp Seq Anal
; 29(7): 1015-1024, 2018 10.
Article
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| MEDLINE
| ID: mdl-29157056
ABSTRACT
Fungal mitochondrial genes are frequently noted for the presence of introns. These introns are self-splicing and can be assigned to either group I or II introns and they can encode open reading frames (ORFs). This study examines the introns present within the cytochrome b (cytb) gene of ascomycetes fungi. Cytochrome b gene sequences were sampled from GenBank and supplemented with our own data for species of Leptographium and Ophiostoma. Group I introns were encountered most frequently, many encoding either LAGLIDADG or GIY-YIG homing endonucleases (HEs). Numerous examples of different intron/ORF arrangements were observed including nested ORFs, multiple ORFs within a single intron and intron ORFs at various stages of erosion due to the accumulation of mutations. In addition, we noted one example of a nested intron and one complex group II intron that could potentially allow for alternative splicing. Documenting the distribution of introns within the same gene across a range of species allows for a better understanding of the evolution of introns and intronic ORFs. Intron landscapes also are a resource that can help in annotating genes and in bioprospecting for potentially active HEs, which are rare-cutting DNA endonucleases with applications in biotechnology.
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Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Ascomicetos
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Proteínas Fúngicas
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Intrones
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Sistemas de Lectura Abierta
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Citocromos b
Idioma:
En
Año:
2018
Tipo del documento:
Article