Visualizing translational errors: one cell at a time.
Curr Genet
; 64(3): 551-554, 2018 Jun.
Article
en En
| MEDLINE
| ID: mdl-29159424
ABSTRACT
Physiological heterogeneity among single cells with identical genetic information has been observed in a large number of bacterial phenotypes, including growth, stress responses, cell size, and antibiotic tolerance. Despite the widespread observation of this phenomenon in bacterial populations, not much is known about the molecular mechanisms behind phenotypic heterogeneity. Currently, our understanding is primarily limited to transcriptional profile of single cells using fluorescence reporters. Although the development of these tools has been extremely informative, it cannot fully explain the heterogeneity seen in populations. In a recent publication, Fan et al. have developed a dual-fluorescent reporter system that is capable of quantitatively measuring translational fidelity in single cells. It is shown that translational fidelity is heterogeneous and affects the growth characteristics of single cells. The development of tools for analysis of molecular heterogeneity downstream of transcription may play an important role in advancing our understanding of the physiology of bacterial populations.
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Banco de datos:
MEDLINE
Asunto principal:
Biosíntesis de Proteínas
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Heterogeneidad Genética
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Análisis de la Célula Individual
Idioma:
En
Año:
2018
Tipo del documento:
Article