Beating Bias in the Directed Evolution of Proteins: Combining High-Fidelity on-Chip Solid-Phase Gene Synthesis with Efficient Gene Assembly for Combinatorial Library Construction.

Li, Aitao; Acevedo-Rocha, Carlos G; Sun, Zhoutong; Cox, Tony; Xu, Jia Lucy; Reetz, Manfred T

Li, Aitao; Acevedo-Rocha, Carlos G; Sun, Zhoutong; Cox, Tony; Xu, Jia Lucy; Reetz, Manfred T.

Li A; Department of Synthetic Organic Chemistry, Max-Planck-Institut für Kohlenforschung, Kaiser-Wilhelm-Platz 1, 45470, Muelheim, Germany.
Acevedo-Rocha CG; Department of Chemistry, Philipps-Universität Marburg, 35032, Marburg, Germany.
Sun Z; Hubei Collaborative Innovation Center for, Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, 368 Youyi Road, Wuchang Wuhan, 430062, P.R. China.
Cox T; Biosyntia ApS, 2100, Copenhagen, Denmark.
Xu JL; Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin, 300308, P.R. China.
Reetz MT; Twist Bioscience, 455 Mission Bay Boulevard South, San Francisco, CA, 94158, USA.

Chembiochem ; 19(3): 221-228, 2018 02 02.

Article en En | MEDLINE | ID: mdl-29171900

Asunto(s)

Proteínas Bacterianas/genética; Técnicas Químicas Combinatorias; ADN/genética; Evolución Molecular Dirigida; Epóxido Hidrolasas/genética; Proteínas Bacterianas/metabolismo; Biocatálisis; Epóxido Hidrolasas/metabolismo; Estructura Molecular; Rhodococcus/enzimología; Silicio/química

Palabras clave

directed evolution; enzymes; polymerase chain reaction; saturation mutagenesis; solid-phase synthesis

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Proteínas Bacterianas / ADN / Evolución Molecular Dirigida / Técnicas Químicas Combinatorias / Epóxido Hidrolasas Tipo de estudio: Clinical_trials Idioma: En Año: 2018 Tipo del documento: Article

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