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Large-Scale Femtoliter Droplet Array for Single Cell Efflux Assay of Bacteria.
Iino, Ryota; Sakakihara, Shouichi; Matsumoto, Yoshimi; Nishino, Kunihiko.
  • Iino R; Okazaki Institute for Integrative Bioscience, Institute for Molecular Science, National Institutes of Natural Sciences, Okazaki, Aichi, 444-8787, Japan. iino@ims.ac.jp.
  • Sakakihara S; Department of Functional Molecular Science, School of Physical Sciences, The Graduate University for Advanced Studies (SOKENDAI), Kanagawa, 240-0193, Japan. iino@ims.ac.jp.
  • Matsumoto Y; Technical Division, Institute of Scientific and Industrial Research, Osaka University, Osaka, Japan.
  • Nishino K; Department of Biomolecular Science and Regulation, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka, Japan.
Methods Mol Biol ; 1700: 331-341, 2018.
Article en En | MEDLINE | ID: mdl-29177839
ABSTRACT
Large-scale femtoliter droplet array as a platform for single cell efflux assay of bacteria is described. Device microfabrication, femtoliter droplet array formation and concomitant enclosure of single bacterial cells, fluorescence-based detection of efflux activity at the single cell level, and collection of single cells from droplet and subsequent gene analysis are described in detail.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Proteínas de Transporte de Membrana / Análisis de Matrices Tisulares / Análisis de la Célula Individual Idioma: En Año: 2018 Tipo del documento: Article
Texto completo
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Proteínas de Transporte de Membrana / Análisis de Matrices Tisulares / Análisis de la Célula Individual Idioma: En Año: 2018 Tipo del documento: Article