Quinalizarin exerts an anti-tumour effect on lung cancer A549 cells by modulating the Akt, MAPK, STAT3 and p53 signalling pathways.
Mol Med Rep
; 17(2): 2626-2634, 2018 Feb.
Article
en En
| MEDLINE
| ID: mdl-29207064
ABSTRACT
Quinalizarin may be a potential chemical agent for cancer therapy, as it exerts antitumour effects against a variety of different types of cancer. However, the underlying regulatory mechanism and signalling pathways of quinalizarin in lung cancer cells remains unknown. The present study sought to investigate the effects of quinalizarin on proliferation, apoptosis and reactive oxygen species (ROS) generation in lung cancer. MTT assays were used to evaluate the effects of quinalizarin on the viability of lung cancer A549, NCIH460 and NCIH23 cells. Flow cytometry was employed to evaluate the effects of quinalizarin on the cell cycle, apoptosis and ROS generation in A549 cells. Western blotting was performed to detect cell cycle and apoptosisassociated protein expression levels in A549 cells. Quinalizarin inhibited A549, NCIH460 and NCIH23 cell proliferation and induced A549 cell cycle arrest at the G0/G1 phase. Quinalizarin induced apoptosis by upregulating the expression of Bcell lymphoma 2 (Bcl2)associated agonist of cell death, cleavedcaspase3 and cleavedpoly (adenosine diphosphateribose) polymerase, and downregulating the expression of Bcl2. Furthermore, quinalizarin activated mitogenactivated protein kinase (MAPK) and p53, and inhibited the protein kinase B and signal transducer and activator of transcription3 (STAT3) signalling pathways. In addition, quinalizarin increased ROS generation. The ROS scavenger NacetylLcysteine restored quinalizarininduced cell apoptosis, and inactivated the MAPK and STAT3 signalling pathways. The results of the present study demonstrated that quinalizarin induces G0/G1 phase cell cycle arrest and apoptosis via ROS mediatedMAPK and STAT3 signalling pathways.
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1
Banco de datos:
MEDLINE
Asunto principal:
Transducción de Señal
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Proteína p53 Supresora de Tumor
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Antraquinonas
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Proteínas Quinasas Activadas por Mitógenos
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Proteínas Proto-Oncogénicas c-akt
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Factor de Transcripción STAT3
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Antineoplásicos
Límite:
Humans
Idioma:
En
Año:
2018
Tipo del documento:
Article