Combined effects of ovariectomy and streptozotocin-induced diabetes in the articular cartilage of rats.
Climacteric
; 21(1): 75-81, 2018 02.
Article
en En
| MEDLINE
| ID: mdl-29231060
ABSTRACT
AIM:
To evaluate the combined effects of streptozotocin-induced diabetes (Di) and ovariectomy in the articular cartilage of rats.METHODS:
Forty adult female Wistar rats were ovariectomized (OVX) or sham-operated. After recovery from surgery, the animals were assigned randomly into four groups OVX control (OVX-C); OVX treated with 10 µg/kg/day of 17ß-estradiol (OVX-E); sham-operated subjected to Di (Sham-Di); and OVX subjected to Di (OVX-Di). After 60 days of treatment, the animals were euthanized and the distal femurs with articular cartilage were processed for paraffin-embedding. Sections were stained with hematoxylin and eosin for histomorphometry, Picro-Sirius Red for collagen, or Alcian Blue for glycosaminoglycan (GAG) content. To detect apoptosis, sections were stained with an antibody to cleaved caspase-3 (casp-3).RESULTS:
Articular cartilage thickness and GAG content were significantly lower (p < 0.05) in the OVX-Di group, which also showed a higher number of casp-3-positive chondrocytes than the other groups. Interestingly, the higher percentage (p < 0.05) of mature collagen fibers was seen in the OVX-Di group, may be as a result of a reduced extracellular matrix remodeling of the articular cartilage.CONCLUSION:
Our results indicate that the combination of ovariectomy and streptozotocin-induced diabetes produces more deleterious effects in articular cartilage of rats than either condition alone.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Ovariectomía
/
Cartílago Articular
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Diabetes Mellitus Experimental
Límite:
Animals
Idioma:
En
Año:
2018
Tipo del documento:
Article