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Identification of tumor-reactive B cells and systemic IgG in breast cancer based on clonal frequency in the sentinel lymph node.
McDaniel, Jonathan R; Pero, Stephanie C; Voss, William N; Shukla, Girja S; Sun, Yujing; Schaetzle, Sebastian; Lee, Chang-Han; Horton, Andrew P; Harlow, Seth; Gollihar, Jimmy; Ellefson, Jared W; Krag, Christopher C; Tanno, Yuri; Sidiropoulos, Nikoletta; Georgiou, George; Ippolito, Gregory C; Krag, David N.
  • McDaniel JR; Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, USA.
  • Pero SC; Department of Surgery, Vermont Cancer Center, University of Vermont Larner College of Medicine, 89 Beaumont Avenue, Given Medical Building, Burlington, VT, 05405, USA.
  • Voss WN; Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, USA.
  • Shukla GS; Department of Surgery, Vermont Cancer Center, University of Vermont Larner College of Medicine, 89 Beaumont Avenue, Given Medical Building, Burlington, VT, 05405, USA.
  • Sun Y; Department of Surgery, Vermont Cancer Center, University of Vermont Larner College of Medicine, 89 Beaumont Avenue, Given Medical Building, Burlington, VT, 05405, USA.
  • Schaetzle S; Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, USA.
  • Lee CH; Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, USA.
  • Horton AP; Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, USA.
  • Harlow S; Department of Surgery, Vermont Cancer Center, University of Vermont Larner College of Medicine, 89 Beaumont Avenue, Given Medical Building, Burlington, VT, 05405, USA.
  • Gollihar J; Department of Molecular Biosciences, The University of Texas at Austin, 100 E. 24th Street, Stop A5000, Austin, TX, 78712, USA.
  • Ellefson JW; Department of Molecular Biosciences, The University of Texas at Austin, 100 E. 24th Street, Stop A5000, Austin, TX, 78712, USA.
  • Krag CC; Department of Surgery, Vermont Cancer Center, University of Vermont Larner College of Medicine, 89 Beaumont Avenue, Given Medical Building, Burlington, VT, 05405, USA.
  • Tanno Y; Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, USA.
  • Sidiropoulos N; Department of Pathology and Laboratory Medicine, University of Vermont Larner College of Medicine, Burlington, VT, USA.
  • Georgiou G; Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, USA.
  • Ippolito GC; Department of Molecular Biosciences, The University of Texas at Austin, 100 E. 24th Street, Stop A5000, Austin, TX, 78712, USA.
  • Krag DN; Department of Molecular Biosciences, The University of Texas at Austin, 100 E. 24th Street, Stop A5000, Austin, TX, 78712, USA. gci@mail.utexas.edu.
Cancer Immunol Immunother ; 67(5): 729-738, 2018 May.
Article en En | MEDLINE | ID: mdl-29427082
ABSTRACT
A better understanding of antitumor immune responses is the key to advancing the field of cancer immunotherapy. Endogenous immunity in cancer patients, such as circulating anticancer antibodies or tumor-reactive B cells, has been historically yet incompletely described. Here, we demonstrate that tumor-draining (sentinel) lymph node (SN) is a rich source for tumor-reactive B cells that give rise to systemic IgG anticancer antibodies circulating in the bloodstream of breast cancer patients. Using a synergistic combination of high-throughput B-cell sequencing and quantitative immunoproteomics, we describe the prospective identification of tumor-reactive SN B cells (based on clonal frequency) and also demonstrate an unequivocal link between affinity-matured expanded B-cell clones in the SN and antitumor IgG in the blood. This technology could facilitate the discovery of antitumor antibody therapeutics and conceivably identify novel tumor antigens. Lastly, these findings highlight the unique and specialized niche the SN can fill in the advancement of cancer immunotherapy.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Neoplasias de la Mama / Inmunoglobulina G / Linfocitos B / Células Clonales / Ganglio Linfático Centinela / Anticuerpos Monoclonales / Antígenos de Neoplasias Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Female / Humans Idioma: En Año: 2018 Tipo del documento: Article
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Neoplasias de la Mama / Inmunoglobulina G / Linfocitos B / Células Clonales / Ganglio Linfático Centinela / Anticuerpos Monoclonales / Antígenos de Neoplasias Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Female / Humans Idioma: En Año: 2018 Tipo del documento: Article