Application of in Vitro T Cell Assay Using Human Leukocyte Antigen-Typed Healthy Donors for the Assessment of Drug Immunogenicity.

ABSTRACT

It is unclear whether priming of naïve T cells to drugs is detectable in healthy human donors expressing different human leukocyte antigen (HLA) alleles. Thus, we examined T cell priming with drugs associated with HLA risk alleles and control compounds in 14 HLA-typed donors. Nitroso sulfamethoxazole and piperacillin activated T cells from all donors, whereas responses to carbamazepine and oxypurinol were only seen in donors expressing HLA-B*1502 and HLA-B*5801, respectively. Weak flucloxacillin-specific T cell responses were detected in donors expressing HLA-B*5701 and HLA-B*5801. These data show that the priming of T cells with certain drugs is skewed toward donors expressing specific HLA alleles.