Differential expression of proteome in aqueous humor in patients with and without glaucoma. / Expresión diferencial del proteoma en humor acuoso de pacientes con y sin glaucoma.
Arch Soc Esp Oftalmol (Engl Ed)
; 93(4): 160-168, 2018 Apr.
Article
en En, Es
| MEDLINE
| ID: mdl-29439810
ABSTRACT
OBJECTIVE:
To determine quantitative and qualitative differences of aqueous humor proteome in patients with and without glaucoma.METHOD:
Observational, descriptive and cross-sectional study of 12 patients (8 men; 4 women) with and without glaucoma. There are 3 groups of minority proteins with serum equimolar contribution of each of the patients. Specimens were obtained during cataract surgery from patients without glaucoma (performed with retrobulbar anaesthesia [cataract retrobulbar patient -CRP-;n=4] or topical [cataract topical patient -CTP-; n=4]), or from patients with glaucoma (performed with retrobulbar anaesthesia [glaucoma retrobulbar patient -GRP-; n=4]). The humor proteome samples were frozen at -80°C until processing by trypsin digestion to obtain tryptic peptides, and then performing liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) to obtain the proteome and its differential expression between groups. Statistical analysis was performed using the SPSS v.17 program.RESULTS:
The study included 12 patients, aged (mean±standard deviation) 74.50±9.53 years. Concentrations obtained 0.48±0.25µg/µl for CRP, 0.28±0.04µg/µl for CTP, and 0.35±0.16µg/µl for GRP. A total of 309 proteins were identified, of which 205, 210, and 182 were in CRP, CTP, and GRP, respectively. A total of 114 proteins were common to all three groups, 50 were exclusive to CRP, 58 to CTP, and 27 to GRP.CONCLUSIONS:
In this pilot study, a quantitative difference was found in the protein expression of humor among patients with glaucoma, there being 27 proteins unique to patients with glaucomatous disease.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Humor Acuoso
/
Glaucoma
/
Proteoma
Tipo de estudio:
Observational_studies
/
Prevalence_studies
/
Qualitative_research
/
Risk_factors_studies
Límite:
Aged
/
Aged80
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Female
/
Humans
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Male
/
Middle aged
Idioma:
En
/
Es
Año:
2018
Tipo del documento:
Article