Highly Effective and Low-Cost MicroRNA Detection with CRISPR-Cas9.
ACS Synth Biol
; 7(3): 807-813, 2018 03 16.
Article
en En
| MEDLINE
| ID: mdl-29486117
ABSTRACT
MicroRNAs have been reported as related to multiple diseases and have potential applications in diagnosis and therapeutics. However, detection of miRNAs remains improvable, given their complexity, high cost, and low sensitivity as of currently. In this study, we attempt to build a novel platform that detects miRNAs at low cost and high efficacy. This detection system contains isothermal amplification, detecting and reporting process based on rolling circle amplification, CRISPR-Cas9, and split-horseradish peroxidase techniques. It is able to detect trace amount of miRNAs from samples with mere single-base specificity. Moreover, we demonstrated that such scheme can effectively detect target miRNAs in clinical serum samples and significantly distinguish patients of non-small cell lung cancer from healthy volunteers by detecting the previously reported biomarker circulating let-7a. As the first to use CRISPR-Cas9 in miRNA detection, this method is a promising approach capable of being applied in screening, diagnosing, and prognosticating of multiple diseases.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Técnicas Genéticas
/
Costos y Análisis de Costo
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MicroARNs
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Sistemas CRISPR-Cas
Tipo de estudio:
Diagnostic_studies
/
Health_economic_evaluation
Límite:
Humans
Idioma:
En
Año:
2018
Tipo del documento:
Article