Induced pluripotent stem cells-derived myeloid-derived suppressor cells regulate the CD8+ T cell response.
Stem Cell Res
; 29: 32-41, 2018 05.
Article
en En
| MEDLINE
| ID: mdl-29574174
ABSTRACT
Myeloid-derived suppressor cells (MDSCs) are markedly increased in cancer patients and tumor-bearing mice and promote tumor growth and survival by inhibiting host innate and adaptive immunity. In this study, we generated and characterized MDSCs from murine-induced pluripotent stem cells (iPSCs). The iPSCs were co-cultured with OP9 cells, stimulated with GM-CSF, and became morphologically heterologous under co-culturing with hepatic stellate cells. Allogeneic and OVA-specific antigen stimulation demonstrated that iPS-MDSCs have a T-cell regulatory function. Furthermore, a popliteal lymph node assay and autoimmune hepatitis model showed that iPS-MDSCs also regulate immune responsiveness in vivo and have a therapeutic effect against hepatitis. Taken together, our results demonstrated a method of generating functional MDSCs from iPSCs and highlighted the potential of iPS-MDSCs as a key cell therapy resource for transplantation and autoimmune diseases.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Linfocitos T CD8-positivos
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Células Madre Pluripotentes Inducidas
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Células Supresoras de Origen Mieloide
Límite:
Animals
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Humans
Idioma:
En
Año:
2018
Tipo del documento:
Article