An avirulent Micropterus salmoides rhabdovirus vaccine candidate protects Chinese perch against rhabdovirus infection.
Fish Shellfish Immunol
; 77: 474-480, 2018 Jun.
Article
en En
| MEDLINE
| ID: mdl-29604344
ABSTRACT
In order to develop live vaccine against Siniperca chuatsi rhabdovirus (SCRV) disease, an avirulent virus strain, designed as Micropterus salmoides rhabdovirus Sanshui (MSRV-SS), was selected from six fish rhabdovirus isolates (SCRV-QYãSCRV-SSãSCRV-GMãCMRV-FSãOMBRV-JMãMSRV-SS) by fish challenge assay. When Chinese perch (Siniperca chuatsi) were intraperitoneally injected live virus strain MSRV-SS, they were completely protected from virulent SCRV-GM challenge with a relative percent survival (RPS) of 100% on 18th day post vaccination. Then, the wild type MSRV-SS was purified by plaque clone assays, and the biological characteristics of the clonal strain designed as MSRV-SS-7 were investigated. The MSRV-SS-7 was avirulent to Chinese perch and its growth characteristic was similar to the MSRV-SS. The immune protection effects of clonal MSRV-SS-7 against virulent SCRV-GM were evaluated by intraperitoneal injection (IP) vaccination and immersion (IM) vaccination, their RPSs were all 100%. Altogether, these results indicate that MSRV-SS-7 is a potential live vaccine candidate against SCRV disease.
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MEDLINE
Asunto principal:
Rhabdoviridae
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Perciformes
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Vacunas Virales
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Vacunación
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Infecciones por Rhabdoviridae
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Enfermedades de los Peces
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Animals
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En
Año:
2018
Tipo del documento:
Article