A two dose immunization with an inactivated reassortant H5N2 virus protects chickens against lethal challenge with homologous 2.3.2.1 clade and heterologous 2.2 clade highly pathogenic avian influenza H5N1 viruses.
Vet Microbiol
; 217: 149-157, 2018 Apr.
Article
en En
| MEDLINE
| ID: mdl-29615248
ABSTRACT
The present study was aimed at generating a reassortant vaccine candidate virus with clade 2.3.2.1 Hemagglutinin (HA) and its evaluation in a challenge study for protection against homologous (2.3.2.1 clade) and heterologous (2.2 clade) highly pathogenic avian influenza (HPAI) H5N1 viruses. Plasmid-based reverse genetics technique was used to rescue a 5â¯+â¯3 reassortant H5N2 strain containing the modified HA of H5N1 (clade 2.3.2.1), the Neuraminidase (NA) of H9N2, the Matrix (M) of H5N1 and the internal genes of A/WSN/33 H1N1. In addition, another 6â¯+â¯2 reassortant virus containing modified HA from H5N1 (clade 2.3.2.1), the NA from H9N2 and the internal genes of A/WSN/33 H1N1 was also rescued. The 5â¯+â¯3 reassortant H5N2 virus could grow to a higher titer in both MDCK cells and chicken eggs compared to the 6â¯+â¯2 reassortant H5N2 virus. The vaccine containing the inactivated 5â¯+â¯3 reassortant H5N2 virus was used in a two-dose immunization regime which protected specific pathogen free (SPF) chickens against two repeated challenges with homologous 2.3.2.1 clade and heterologous 2.2 clade HPAI H5N1 viruses. The 5â¯+â¯3 reassortant H5N2 virus based on clade 2.3.2.1 generated in this study can be effective in protecting chickens in the case of an outbreak caused by antigenically different clade 2.2 HPAI H5N1 viruses and opens the way to explore its applicability as potential vaccine candidate especially in the Asian countries reporting these clades frequently. The study also indicates that sequential immunization can broaden protection level against antigenically diverse strains of H5N1 viruses.
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Banco de datos:
MEDLINE
Asunto principal:
Vacunas contra la Influenza
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Vacunas de Productos Inactivados
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Inmunización
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Subtipo H5N1 del Virus de la Influenza A
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Subtipo H5N2 del Virus de la Influenza A
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Gripe Aviar
Límite:
Animals
Idioma:
En
Año:
2018
Tipo del documento:
Article