A highly sensitive electrochemical biosensor for phenol derivatives using a graphene oxide-modified tyrosinase electrode.
Bioelectrochemistry
; 122: 174-182, 2018 Aug.
Article
en En
| MEDLINE
| ID: mdl-29656242
ABSTRACT
The fabrication, characterization and analytical performance were investigated for a phenol biosensor based on the covalent bonding of tyrosinase (TYR) onto a graphene oxide (GO)-modified glassy carbon electrode (GCE) via glutaraldehyde (GA). The surface morphology of the modified electrode was studied by atomic force microscope (AFM) and field-emission scanning electron microscopy (FE-SEM). The fabricated TYR/GA/GO/GCE biosensor showed very good stability, reproducibility, sensitivity and practical usage. The catechol biosensor exhibited a wide sensing linear range from 5×10-8M to 5×10-5M, a lower detection limit of 3×10-8M, a current maximum (Imax) of 65.8µA and an apparent Michaelis constant (Kmapp) of 169.9µM.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Fenoles
/
Técnicas Biosensibles
/
Monofenol Monooxigenasa
/
Agaricales
/
Grafito
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Año:
2018
Tipo del documento:
Article