The endogenous agonist, ß-alanine, activates glycine receptors in rat spinal dorsal neurons.
Biochem Biophys Res Commun
; 500(4): 897-901, 2018 06 12.
Article
en En
| MEDLINE
| ID: mdl-29702090
ABSTRACT
ß-alanine is a structural analog of glycine and γ-aminobutyric acid (GABA) and is thought to be involved in the modulation of nociceptive information at the spinal cord. However, it is not known whether ß-alanine exerts its effect in substantia gelatinosa (SG) neurons of the spinal dorsal horn, where glycine and GABA play an important role in regulating nociceptive transmission from the periphery. Here, we investigated the effects of ß-alanine on inhibitory synaptic transmission in adult rat SG neurons using whole-cell patch-clamp. ß-alanine dose-dependently induced outward currents in SG neurons. Current-voltage plots revealed a reversal potential at approximately -70â¯mV, which was close to the equilibrium potential of Cl-. Pharmacological analysis revealed that ß-alanine activates glycine receptors, but not GABAA receptors. These results suggest that ß-alanine hyperpolarizes the membrane potential of SG neurons by activating Cl- channels through glycine receptors. Our findings raise the possibility that ß-alanine may modulate pain sensation through glycine receptors.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Sustancia Gelatinosa
/
Beta-Alanina
/
Receptores de Glicina
/
Transmisión Sináptica
/
Células del Asta Posterior
/
Nocicepción
Límite:
Animals
Idioma:
En
Año:
2018
Tipo del documento:
Article