Quercetin in equine frozen semen.
Cryo Letters
; 38(4): 299-304, 2017.
Article
en En
| MEDLINE
| ID: mdl-29734431
ABSTRACT
ããBACKGROUND:
Supplementation of sperm diluents to reduce the damage caused by the freeze-thaw cycle is broadly used in equine semen cryopreservation.OBJECTIVE:
The present study aimed at determining the most appropriate quercetin supplementation in equine freezing extender. MATERIALS ANDMETHODS:
Quercetin at four different concentrations (0.25, 0.5, 0.75 or 1 mM) was added in the sperm freezing diluent before the freeze-thaw cycle. The spermatozoa population was analyzed by flow cytometry and a statistical analysis was conducted to detect significant differences between control and treated samples.RESULTS:
The statistical analysis did not reveal any significant modification of seminal parameters.CONCLUSION:
Within the concentrations tested, quercetin supplementation in equine freezing extender did not affect progressive motility, mitochondrial functionality, acrosome reaction, membrane integrity or DNA fragmentation index in post-thaw equine semen.
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Banco de datos:
MEDLINE
Asunto principal:
Quercetina
/
Preservación de Semen
/
Caballos
Límite:
Animals
Idioma:
En
Año:
2017
Tipo del documento:
Article