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High-speed volumetric imaging of neuronal activity in freely moving rodents.
Skocek, Oliver; Nöbauer, Tobias; Weilguny, Lukas; Martínez Traub, Francisca; Xia, Chuying Naomi; Molodtsov, Maxim I; Grama, Abhinav; Yamagata, Masahito; Aharoni, Daniel; Cox, David D; Golshani, Peyman; Vaziri, Alipasha.
  • Skocek O; Laboratory of Neurotechnology and Biophysics, The Rockefeller University, New York, NY, USA.
  • Nöbauer T; Laboratory of Neurotechnology and Biophysics, The Rockefeller University, New York, NY, USA.
  • Weilguny L; Research Institute of Molecular Pathology, Vienna, Austria.
  • Martínez Traub F; Laboratory of Neurotechnology and Biophysics, The Rockefeller University, New York, NY, USA.
  • Xia CN; Laboratory of Neurotechnology and Biophysics, The Rockefeller University, New York, NY, USA.
  • Molodtsov MI; Research Institute of Molecular Pathology, Vienna, Austria.
  • Grama A; Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, USA.
  • Yamagata M; Center for Brain Science, Harvard University, Cambridge, MA, USA.
  • Aharoni D; Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, USA.
  • Cox DD; Center for Brain Science, Harvard University, Cambridge, MA, USA.
  • Golshani P; Department of Neurology, Integrative Center for Learning and Memory, Brain Research Institute, University of California, Los Angeles, Los Angeles, CA, USA.
  • Vaziri A; Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, USA.
Nat Methods ; 15(6): 429-432, 2018 06.
Article en En | MEDLINE | ID: mdl-29736000
ABSTRACT
Thus far, optical recording of neuronal activity in freely behaving animals has been limited to a thin axial range. We present a head-mounted miniaturized light-field microscope (MiniLFM) capable of capturing neuronal network activity within a volume of 700 × 600 × 360 µm3 at 16 Hz in the hippocampus of freely moving mice. We demonstrate that neurons separated by as little as ~15 µm and at depths up to 360 µm can be discriminated.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Hipocampo / Miniaturización / Neuronas Límite: Animals Idioma: En Año: 2018 Tipo del documento: Article
Texto completo
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XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Hipocampo / Miniaturización / Neuronas Límite: Animals Idioma: En Año: 2018 Tipo del documento: Article