Cross-Linked Fluorescent Supramolecular Nanoparticles for Intradermal Controlled Release of Antifungal Drug-A Therapeutic Approach for Onychomycosis.
ACS Nano
; 12(7): 6851-6859, 2018 07 24.
Article
en En
| MEDLINE
| ID: mdl-29851454
ABSTRACT
The existing approaches to onychomycosis demonstrate limited success since the commonly used oral administration and topical cream only achieve temporary effective drug concentration at the fungal infection sites. An ideal therapeutic approach for onychomycosis should have (i) the ability to introduce antifungal drugs directly to the infected sites; (ii) finite intradermal sustainable release to maintain effective drug levels over prolonged time; (iii) a reporter system for monitoring maintenance of drug level; and (iv) minimum level of inflammatory responses at or around the fungal infection sites. To meet these expectations, we introduced ketoconazole-encapsulated cross-linked fluorescent supramolecular nanoparticles (KTZâc-FSMNPs) as an intradermal controlled release solution for treating onychomycosis. A two-step synthetic approach was adopted to prepare a variety of KTZâc-FSMNPs. Initial characterization revealed that 4800 nm KTZâc-FSMNPs exhibited high KTZ encapsulation efficiency/capacity, optimal fluorescent property, and sustained KTZ release profile. Subsequently, 4800 nm KTZâc-FSMNPs were chosen for in vivo studies using a mouse model, wherein the KTZâc-FSMNPs were deposited intradermally via tattoo. The results obtained from (i) in vivo fluorescence imaging, (ii) high-performance liquid chromatography quantification of residual KTZ, (iii) matrix-assisted laser desorption/ionization mass spectrometry imaging mapping of KTZ distribution in intradermal regions around the tattoo site, and (iv) histology for assessment of local inflammatory responses and biocompatibility, suggest that 4800 nm KTZâc-FSMNPs can serve as an effective treatment for onychomycosis.
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Banco de datos:
MEDLINE
Asunto principal:
Onicomicosis
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Reactivos de Enlaces Cruzados
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Nanopartículas
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Colorantes Fluorescentes
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Dermatosis del Pie
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Cetoconazol
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Antifúngicos
Límite:
Animals
Idioma:
En
Año:
2018
Tipo del documento:
Article