A novel label-free fluorescent sensor for highly sensitive detection of bleomycin based on nitrogen-doped graphene quantum dots.
Anal Chim Acta
; 1028: 45-49, 2018 Oct 22.
Article
en En
| MEDLINE
| ID: mdl-29884352
ABSTRACT
In this work, we presented a novel label-free biosensor for rapid detection of bleomycinsulphate (BLM). The biosensor was based on the fluorescent "turn off-on" of nitrogen-doped graphene quantum dots (N-GQDs), which was prepared in a green way from citric acid and ammonia. The richness of carboxyl groups on the N-GQDs enabled strong adsorption of ssDNA to the surface of N-GQDs through π-π stacking interactions, resulting in the effective fluorescence quenching of N-GQDs system. The ssDNA underwent an irreversible cleavage event via the oxidative effect of BLM with Fe(II) as a cofactor, thus a turn-on fluorescence signal was observed. Thereby, the concentration of BLM can be quantitatively determined in a broad range from 0.34â¯nmol/L to 1300â¯nmol/L with a detection limit of 0.34â¯nmol/L. The presented method was applied to the determination of BLM in human serum samples with satisfactory results.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Bleomicina
/
Técnicas Biosensibles
/
Puntos Cuánticos
/
Límite de Detección
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Grafito
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Nitrógeno
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Año:
2018
Tipo del documento:
Article