Dose-dependency of multiple free radical-scavenging activity of edaravone.
J Surg Res
; 228: 147-153, 2018 08.
Article
en En
| MEDLINE
| ID: mdl-29907205
ABSTRACT
BACKGROUND:
Edaravone is a powerful free radical scavenger that is in clinical use. However, data concerning its dose-response relationship against multiple free radicals remain sparse. The purpose of the present study was to demonstrate the dose-dependency of direct scavenging activity of edaravone against multiple free radical species. MATERIALS ANDMETHODS:
Free radical-scavenging activities of edaravone against six free radical species were evaluated by electron spin resonance spectroscopy using spin-trapping method.RESULTS:
Edaravone scavenged the following free radicals in dose-dependent manners with reaction rate constants (kedaravone) or 50% inhibitory concentration (IC50) as indicated hydroxyl radical (kedaravone = 5.2 × 1010 M-1 s-1), superoxide anion (kedaravone/kG-CYPMPO = 0.63), tert-butyl peroxyl radical (kedaravone/kG-CYPMPO = 8.8), ascorbyl free radical (IC50 = 0.17 ± 0.06 mM), 2,2-diphenyl-1-picrylhydrazyl (DPPH, IC50 = 4.7 ± 0.3 µM), and nitric oxide (kedaravone = 7.0 × 103 M-1 s-1).CONCLUSIONS:
The dose-dependent scavenging activities of edaravone against multiple free radical species were clearly illustrated. It is speculated that edaravone acts as antioxidant by dose-dependently scavenging multiple free radical species along the chain reactions of oxidative stress in surgery.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Depuradores de Radicales Libres
/
Radicales Libres
/
Edaravona
Tipo de estudio:
Etiology_studies
Límite:
Humans
Idioma:
En
Año:
2018
Tipo del documento:
Article