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Comprehensive ADP-ribosylome analysis identifies tyrosine as an ADP-ribose acceptor site.
Leslie Pedrioli, Deena M; Leutert, Mario; Bilan, Vera; Nowak, Kathrin; Gunasekera, Kapila; Ferrari, Elena; Imhof, Ralph; Malmström, Lars; Hottiger, Michael O.
  • Leslie Pedrioli DM; Department of Molecular Mechanisms of Disease, University of Zurich, Zurich, Switzerland.
  • Leutert M; Department of Molecular Mechanisms of Disease, University of Zurich, Zurich, Switzerland.
  • Bilan V; Molecular Life Science PhD Program of the Life Science Zurich Graduate School, Zurich, Switzerland.
  • Nowak K; Department of Molecular Mechanisms of Disease, University of Zurich, Zurich, Switzerland.
  • Gunasekera K; Department of Molecular Mechanisms of Disease, University of Zurich, Zurich, Switzerland.
  • Ferrari E; Molecular Life Science PhD Program of the Life Science Zurich Graduate School, Zurich, Switzerland.
  • Imhof R; Department of Molecular Mechanisms of Disease, University of Zurich, Zurich, Switzerland.
  • Malmström L; Department of Molecular Mechanisms of Disease, University of Zurich, Zurich, Switzerland.
  • Hottiger MO; Department of Molecular Mechanisms of Disease, University of Zurich, Zurich, Switzerland.
EMBO Rep ; 19(8)2018 08.
Article en En | MEDLINE | ID: mdl-29954836
ABSTRACT
Despite recent mass spectrometry (MS)-based breakthroughs, comprehensive ADP-ribose (ADPr)-acceptor amino acid identification and ADPr-site localization remain challenging. Here, we report the establishment of an unbiased, multistep ADP-ribosylome data analysis workflow that led to the identification of tyrosine as a novel ARTD1/PARP1-dependent in vivo ADPr-acceptor amino acid. MS analyses of in vitro ADP-ribosylated proteins confirmed tyrosine as an ADPr-acceptor amino acid in RPS3A (Y155) and HPF1 (Y238) and demonstrated that trans-modification of RPS3A is dependent on HPF1. We provide an ADPr-site Localization Spectra Database (ADPr-LSD), which contains 288 high-quality ADPr-modified peptide spectra, to serve as ADPr spectral references for correct ADPr-site localizations.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Tirosina / Adenosina Difosfato Ribosa / ADP-Ribosilación Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Año: 2018 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Tirosina / Adenosina Difosfato Ribosa / ADP-Ribosilación Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Año: 2018 Tipo del documento: Article