Circular permutation of a bacterial tyrosinase enables efficient polyphenol-specific oxidation and quantitative preparation of orobol.

Lee, Pyung-Gang; Lee, Sang-Hyuk; Hong, Eun Young; Lutz, Stefan; Kim, Byung-Gee

Lee, Pyung-Gang; Lee, Sang-Hyuk; Hong, Eun Young; Lutz, Stefan; Kim, Byung-Gee.

Lee PG; School of Chemical and Biological Engineering, Seoul National University, Seoul, Korea.
Lee SH; Institute of Molecular Biology and Genetics, Seoul National University, Seoul, Korea.
Hong EY; School of Chemical and Biological Engineering, Seoul National University, Seoul, Korea.
Lutz S; Institute of Molecular Biology and Genetics, Seoul National University, Seoul, Korea.
Kim BG; School of Chemical and Biological Engineering, Seoul National University, Seoul, Korea.

Biotechnol Bioeng ; 116(1): 19-27, 2019 01.

Article en En | MEDLINE | ID: mdl-30011063

Asunto(s)

Bacillus megaterium/enzimología; Flavonoides/metabolismo; Monofenol Monooxigenasa/metabolismo; Proteínas Mutantes/metabolismo; Polifenoles/metabolismo; Ingeniería de Proteínas/métodos; Cinética; Monofenol Monooxigenasa/química; Monofenol Monooxigenasa/genética; Proteínas Mutantes/química; Proteínas Mutantes/genética; Oxidación-Reducción; Conformación Proteica

Palabras clave

circular permutation; orobol; polyphenol oxidase; protein engineering; tyrosinase

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Bacillus megaterium / Flavonoides / Ingeniería de Proteínas / Monofenol Monooxigenasa / Proteínas Mutantes / Polifenoles Tipo de estudio: Prognostic_studies Idioma: En Año: 2019 Tipo del documento: Article

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