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Single-cell transcriptomes from human kidneys reveal the cellular identity of renal tumors.
Young, Matthew D; Mitchell, Thomas J; Vieira Braga, Felipe A; Tran, Maxine G B; Stewart, Benjamin J; Ferdinand, John R; Collord, Grace; Botting, Rachel A; Popescu, Dorin-Mirel; Loudon, Kevin W; Vento-Tormo, Roser; Stephenson, Emily; Cagan, Alex; Farndon, Sarah J; Del Castillo Velasco-Herrera, Martin; Guzzo, Charlotte; Richoz, Nathan; Mamanova, Lira; Aho, Tevita; Armitage, James N; Riddick, Antony C P; Mushtaq, Imran; Farrell, Stephen; Rampling, Dyanne; Nicholson, James; Filby, Andrew; Burge, Johanna; Lisgo, Steven; Maxwell, Patrick H; Lindsay, Susan; Warren, Anne Y; Stewart, Grant D; Sebire, Neil; Coleman, Nicholas; Haniffa, Muzlifah; Teichmann, Sarah A; Clatworthy, Menna; Behjati, Sam.
  • Young MD; Wellcome Sanger Institute, Hinxton CB10 1SA, UK.
  • Mitchell TJ; Wellcome Sanger Institute, Hinxton CB10 1SA, UK.
  • Vieira Braga FA; Cambridge University Hospitals NHS Foundation Trust, Cambridge CB2 0QQ, UK.
  • Tran MGB; Department of Surgery, University of Cambridge, Cambridge CB2 0QQ, UK.
  • Stewart BJ; Wellcome Sanger Institute, Hinxton CB10 1SA, UK.
  • Ferdinand JR; UCL Division of Surgery and Interventional Science, Royal Free Hospital, London NW3 2PS, UK.
  • Collord G; Specialist Centre for Kidney Cancer, Royal Free Hospital, London NW3 2PS, UK.
  • Botting RA; Molecular Immunity Unit, Department of Medicine, University of Cambridge, MRC Laboratory of Molecular Biology, Cambridge CB2 0QQ, UK.
  • Popescu DM; Molecular Immunity Unit, Department of Medicine, University of Cambridge, MRC Laboratory of Molecular Biology, Cambridge CB2 0QQ, UK.
  • Loudon KW; Wellcome Sanger Institute, Hinxton CB10 1SA, UK.
  • Vento-Tormo R; Cambridge University Hospitals NHS Foundation Trust, Cambridge CB2 0QQ, UK.
  • Stephenson E; Department of Paediatrics, University of Cambridge, Cambridge CB2 0QQ, UK.
  • Cagan A; Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.
  • Farndon SJ; Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.
  • Del Castillo Velasco-Herrera M; Molecular Immunity Unit, Department of Medicine, University of Cambridge, MRC Laboratory of Molecular Biology, Cambridge CB2 0QQ, UK.
  • Guzzo C; Wellcome Sanger Institute, Hinxton CB10 1SA, UK.
  • Richoz N; Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.
  • Mamanova L; Wellcome Sanger Institute, Hinxton CB10 1SA, UK.
  • Aho T; Wellcome Sanger Institute, Hinxton CB10 1SA, UK.
  • Armitage JN; Great Ormond Street Hospital for Children NHS Foundation Trust, London WC1N 3JH, UK.
  • Riddick ACP; UCL Great Ormond Street Hospital Institute of Child Health, London WC1N 1E, UK.
  • Mushtaq I; Wellcome Sanger Institute, Hinxton CB10 1SA, UK.
  • Farrell S; Wellcome Sanger Institute, Hinxton CB10 1SA, UK.
  • Rampling D; Molecular Immunity Unit, Department of Medicine, University of Cambridge, MRC Laboratory of Molecular Biology, Cambridge CB2 0QQ, UK.
  • Nicholson J; Wellcome Sanger Institute, Hinxton CB10 1SA, UK.
  • Filby A; Cambridge University Hospitals NHS Foundation Trust, Cambridge CB2 0QQ, UK.
  • Burge J; Department of Surgery, University of Cambridge, Cambridge CB2 0QQ, UK.
  • Lisgo S; Department of Surgery, University of Cambridge, Cambridge CB2 0QQ, UK.
  • Maxwell PH; Great Ormond Street Hospital for Children NHS Foundation Trust, London WC1N 3JH, UK.
  • Lindsay S; Cambridge University Hospitals NHS Foundation Trust, Cambridge CB2 0QQ, UK.
  • Warren AY; Great Ormond Street Hospital for Children NHS Foundation Trust, London WC1N 3JH, UK.
  • Stewart GD; Cambridge University Hospitals NHS Foundation Trust, Cambridge CB2 0QQ, UK.
  • Sebire N; Department of Paediatrics, University of Cambridge, Cambridge CB2 0QQ, UK.
  • Coleman N; Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.
  • Haniffa M; Cambridge University Hospitals NHS Foundation Trust, Cambridge CB2 0QQ, UK.
  • Teichmann SA; Human Developmental Biology Resource, Institute of Genetic Medicine, Newcastle University, Newcastle upon Tyne NE1 3BZ, UK.
  • Clatworthy M; Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 0XY, UK.
  • Behjati S; Human Developmental Biology Resource, Institute of Genetic Medicine, Newcastle University, Newcastle upon Tyne NE1 3BZ, UK.
Science ; 361(6402): 594-599, 2018 08 10.
Article en En | MEDLINE | ID: mdl-30093597
ABSTRACT
Messenger RNA encodes cellular function and phenotype. In the context of human cancer, it defines the identities of malignant cells and the diversity of tumor tissue. We studied 72,501 single-cell transcriptomes of human renal tumors and normal tissue from fetal, pediatric, and adult kidneys. We matched childhood Wilms tumor with specific fetal cell types, thus providing evidence for the hypothesis that Wilms tumor cells are aberrant fetal cells. In adult renal cell carcinoma, we identified a canonical cancer transcriptome that matched a little-known subtype of proximal convoluted tubular cell. Analyses of the tumor composition defined cancer-associated normal cells and delineated a complex vascular endothelial growth factor (VEGF) signaling circuit. Our findings reveal the precise cellular identities and compositions of human kidney tumors.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Transcriptoma / Riñón / Neoplasias Renales Tipo de estudio: Prognostic_studies Límite: Adult / Child / Humans Idioma: En Año: 2018 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Transcriptoma / Riñón / Neoplasias Renales Tipo de estudio: Prognostic_studies Límite: Adult / Child / Humans Idioma: En Año: 2018 Tipo del documento: Article