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How Lactobacillus plantarum shapes its transcriptome in response to contrasting habitats.
Filannino, Pasquale; De Angelis, Maria; Di Cagno, Raffaella; Gozzi, Giorgia; Riciputi, Ylenia; Gobbetti, Marco.
  • Filannino P; Department of Soil, Plant and Food Sciences, University of Bari Aldo Moro, Bari, Italy.
  • De Angelis M; Department of Soil, Plant and Food Sciences, University of Bari Aldo Moro, Bari, Italy.
  • Di Cagno R; Faculty of Science and Technology, Free University of Bozen, Italy.
  • Gozzi G; Department of Agricultural and Food Sciences, Alma Mater Studiorum, University of Bologna, Bologna, Italy.
  • Riciputi Y; Department of Agricultural and Food Sciences, Alma Mater Studiorum, University of Bologna, Bologna, Italy.
  • Gobbetti M; Faculty of Science and Technology, Free University of Bozen, Italy.
Environ Microbiol ; 20(10): 3700-3716, 2018 10.
Article en En | MEDLINE | ID: mdl-30094916
ABSTRACT
Triplets of Lactobacullus plantarum strains were isolated from nine contrasting habitats. Without any passage through other culture media, isolation and cultivation were on model media that strictly reproduced the chemical and physical conditions and stressors of the habitats of origin. Here, we demonstrated how L. plantarum regulates and shapes its transcriptome in response to contrasting habitats. Firstly, multivariate clustering analysis of transcriptional data (RNA-Seq), complemented with metabolomics and phenomics, grouped the strains according to the habitats of origin. Subsequently, selected strains from each habitat switched to repeated cultivation on MRS medium and transcriptomes homogenized into a unique cluster. Adaptation to this common medium mainly relied on activation of genes for phage- and prophage-related proteins and transposases. Finally, the comparison of growth across model media and with respect to MRS medium showed that 44% of the overall 3112 gene transcripts changed depending on the specific habitat. Regulation and shaping of transcriptomes mainly concerned carbohydrate acquisition, pyruvate catabolism, proteolytic system and amino acid, lipid and inorganic ion transport and metabolism, with contrasting responses for contrasting habitats. Pathways reconstruction demonstrated how the large genome size of L. plantarum imparts transcriptome and metabolic flexibility as the basic mechanism for a nomadic lifestyle.
Asunto(s)

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Lactobacillus plantarum Idioma: En Año: 2018 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Lactobacillus plantarum Idioma: En Año: 2018 Tipo del documento: Article