Type 1 human T-cell leukemia virus small envelope protein expressed in mouse cells by using a bovine papilloma virus-derived shuttle vector.
Mol Cell Biol
; 5(11): 3320-4, 1985 Nov.
Article
en En
| MEDLINE
| ID: mdl-3018517
ABSTRACT
In an attempt to express the small (transmembrane) envelope protein p21e of type 1 human T-cell leukemia (lymphotrophic) virus (HTLV-1) exclusive of other viral gene products, we have constructed a recombinant plasmid clone (pMBE-1) in a bovine papillomavirus-derived mammalian expression vector. Mouse C127 cells transfected with the pMBE-1 plasmid expressed the introduced HTLV-1 viral gene(s) as demonstrated by Northern blot and indirect immunofluorescence with natural human antisera. The transfected mouse cells were injected into BALB/c mice, and a monoclonal antibody was recovered which specifically recognizes a 21-kilodalton protein present in HTLV-1 virions, indicating that the pMBE-1 plasmid encodes the small envelope protein.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Papillomaviridae
/
Proteínas del Envoltorio Viral
/
Deltaretrovirus
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Papillomavirus Bovino 1
/
Genes
/
Genes Virales
/
Vectores Genéticos
Límite:
Animals
Idioma:
En
Año:
1985
Tipo del documento:
Article