CRAC Channel Components Quantitative Expression (In Tissues and Cell Lines) Using qPCR.
Methods Mol Biol
; 1843: 95-106, 2018.
Article
en En
| MEDLINE
| ID: mdl-30203280
ABSTRACT
Since last decade real-time qPCR has become a routine and robust approach for measuring the expression of genes of interest. Indeed, using qPCR, expression profile analyses are now possible and participate to the understanding of physiological or pathological role of channels such as calcium release-activated channels (CRAC). Initially discovered in lymphocyte T and immunity perturbations, recent studies have highlighted the role of CRAC channels in other pathologies such as cancer. Here we describe a protocol to quantify CRAC components expression, in tissue sample and cell lines, to validate knockdown strategies or identify their roles in physiological and pathological conditions (Hoth and Penner, J Physiol 465359-386, 1993; Hoth and Penner, Nature 355353-356, 1992; and Zweifach and Lewis, Proc Natl Acad Sci U S A 906295-6299, 1993).
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Expresión Génica
/
Activación del Canal Iónico
/
Reacción en Cadena en Tiempo Real de la Polimerasa
/
Canales de Calcio Activados por la Liberación de Calcio
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Año:
2018
Tipo del documento:
Article