[Chemical Constituents of the Endophytic Fungus Rhinocladiella sp. lgt-3 from Tripterygium wilfordii].

Objective: To study the chemical constituents and monoamine oxidase inhibitory activity of Rhinocladiella sp. lgt-3,which was an endophytic fungus isolated from Tripterygium wilfordii. Methods: Compounds were isolated from by various column chromatography. The structures were elucidated by various spectral data. The activity was tested by using 96-well micro-plated method. Results: 10 compounds were isolated and identified as 3,4-dihydro-3,4,8-trihydroxy-1( 2H)-naphthalenone( 1),( 3R,4R)-4-hydroxymellein( 2),O-methylmellein( 3),4-( 2-hydroxybutynoxy) benzoic acid( 4),2,4-dihydroxy-6-(( R)-4-hydroxy-2-oxopentyl)-3-methylbenzaldehyde( 5),( Z)-N-( 4-hydroxystyryl) formamide( 6), aspterric acid( 7),bufotenin( 8),5-hydroxy-N,N-dimethyltryptamine-N-oxide( 9)and 5-methoxy-N,N-dimethyltryptamine( 10). Compounds 1,8 ~ 10 showed moderate anti-monoamine oxidase activity with IC50 values of 32,36,50,56 µmol/L, respectively. Conclusion: Compounds 3 ~ 10 are isolated from Rhinocladiella genus for the first time and the anti-monoamine oxidase activity of compound 1 are reported for the first time.