BAR-encapsulated nanoparticles for the inhibition and disruption of Porphyromonas gingivalis-Streptococcus gordonii biofilms.
J Nanobiotechnology
; 16(1): 69, 2018 Sep 15.
Article
en En
| MEDLINE
| ID: mdl-30219060
ABSTRACT
BACKGROUND:
Porphyromonas gingivalis adherence to oral streptococci is a key point in the pathogenesis of periodontal diseases (Honda in Cell Host Microbe 10423-425, 2011). Previous work in our groups has shown that a region of the streptococcal antigen denoted BAR (SspB Adherence Region) inhibits P. gingivalis/S. gordonii interaction and biofilm formation both in vitro and in a mouse model of periodontitis (Daep et al. in Infect Immun 745756-5762, 2006; Daep et al. in Infect immun 763273-3280, 2008; Daep et al. in Infect Immun 7967-74, 2011). However, high localized concentration and prolonged exposure are needed for BAR to be an effective therapeutic in the oral cavity.METHODS:
To address these challenges, we fabricated poly(lactic-co-glycolic acid) (PLGA) and methoxy-polyethylene glycol PLGA (mPEG-PLGA) nanoparticles (NPs) that encapsulate BAR peptide, and assessed the potency of BAR-encapsulated NPs to inhibit and disrupt in vitro two-species biofilms. In addition, the kinetics of BAR-encapsulated NPs were compared after different durations of exposure in a two-species biofilm model, against previously evaluated BAR-modified NPs and free BAR.RESULTS:
BAR-encapsulated PLGA and mPEG-PLGA NPs potently inhibited biofilm formation (IC50 = 0.7 µM) and also disrupted established biofilms (IC50 = 1.3 µM) in a dose-dependent manner. In addition, BAR released during the first 2 h of administration potently inhibits biofilm formation, while a longer duration of 3 h is required to disrupt pre-existing biofilms.CONCLUSIONS:
These results suggest that BAR-encapsulated NPs provide a potent platform to inhibit (prevent) and disrupt (treat) P. gingivalis/S. gordonii biofilms, relative to free BAR.Palabras clave
Texto completo:
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Banco de datos:
MEDLINE
Asunto principal:
Portadores de Fármacos
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Porphyromonas gingivalis
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Biopelículas
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Nanopartículas
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Streptococcus gordonii
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Antibacterianos
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Antígenos Bacterianos
Límite:
Humans
Idioma:
En
Año:
2018
Tipo del documento:
Article