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Clinically relevant doses of vitamin A decrease cortical bone mass in mice
Lionikaite, Vikte; Gustafsson, Karin L; Westerlund, Anna; Windahl, Sara H; Koskela, Antti; Tuukkanen, Juha; Johansson, Helena; Ohlsson, Claes; Conaway, H Herschel; Henning, Petra; Lerner, Ulf H.
  • Lionikaite V; Centre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute for Medicine, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden
  • Gustafsson KL; Centre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute for Medicine, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden
  • Westerlund A; Centre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute for Medicine, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden
  • Windahl SH; Centre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute for Medicine, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden
  • Koskela A; Department of Anatomy and Cell Biology, Medical Research Center, University of Oulu, Oulu, Finland
  • Tuukkanen J; Department of Anatomy and Cell Biology, Medical Research Center, University of Oulu, Oulu, Finland
  • Johansson H; Institute for Health and Aging, Catholic University of Australia, Melbourne, Australia
  • Ohlsson C; Centre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute for Medicine, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden
  • Conaway HH; Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA
  • Henning P; Centre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute for Medicine, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden
  • Lerner UH; Centre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute for Medicine, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden
J Endocrinol ; 239(3): 389-402, 2018 10 31.
Article en En | MEDLINE | ID: mdl-30388359
Excess vitamin A has been associated with decreased cortical bone thickness and increased fracture risk. While most studies in rodents have employed high dosages of vitamin A for short periods of time, we investigated the bone phenotype in mice after longer exposure to more clinically relevant doses. For 1, 4 and 10 weeks, mice were fed a control diet (4.5 µg retinyl acetate/g chow), a diet modeled from the human upper tolerable limit (UTL; 20 µg retinyl acetate/g chow) and a diet three times UTL (supplemented; 60 µg retinyl acetate/g chow). Time-dependent decreases in periosteal circumference and bone mineral content were noted with the supplemented dose. These reductions in cortical bone resulted in a significant time-dependent decrease of predicted strength and a non-significant trend toward reduced bone strength as analyzed by three-point bending. Trabecular bone in tibiae and vertebrae remained unaffected when vitamin A was increased in the diet. Dynamic histomorphometry demonstrated that bone formation was substantially decreased after 1 week of treatment at the periosteal site with the supplemental dose. Increasing amount of vitamin A decreased endocortical circumference, resulting in decreased marrow area, a response associated with enhanced endocortical bone formation. In the presence of bisphosphonate, vitamin A had no effect on cortical bone, suggesting that osteoclasts are important, even if effects on bone resorption were not detected by osteoclast counting, genes in cortical bone or analysis of serum TRAP5b and CTX. In conclusion, our results indicate that even clinically relevant doses of vitamin A have a negative impact on the amount of cortical bone.
Asunto(s)

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Osteogénesis / Vitamina A / Hipervitaminosis A / Hueso Cortical Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Año: 2018 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Osteogénesis / Vitamina A / Hipervitaminosis A / Hueso Cortical Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Año: 2018 Tipo del documento: Article