Potassium channel-based optogenetic silencing.
Nat Commun
; 9(1): 4611, 2018 11 05.
Article
en En
| MEDLINE
| ID: mdl-30397200
ABSTRACT
Optogenetics enables manipulation of biological processes with light at high spatio-temporal resolution to control the behavior of cells, networks, or even whole animals. In contrast to the performance of excitatory rhodopsins, the effectiveness of inhibitory optogenetic tools is still insufficient. Here we report a two-component optical silencer system comprising photoactivated adenylyl cyclases (PACs) and the small cyclic nucleotide-gated potassium channel SthK. Activation of this 'PAC-K' silencer by brief pulses of low-intensity blue light causes robust and reversible silencing of cardiomyocyte excitation and neuronal firing. In vivo expression of PAC-K in mouse and zebrafish neurons is well tolerated, where blue light inhibits neuronal activity and blocks motor responses. In combination with red-light absorbing channelrhodopsins, the distinct action spectra of PACs allow independent bimodal control of neuronal activity. PAC-K represents a reliable optogenetic silencer with intrinsic amplification for sustained potassium-mediated hyperpolarization, conferring high operational light sensitivity to the cells of interest.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Canales de Potasio
/
Elementos Silenciadores Transcripcionales
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Optogenética
Límite:
Animals
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Humans
Idioma:
En
Año:
2018
Tipo del documento:
Article