Bacterial indoleacetic acid-induced synthesis of colloidal Ag2O nanocrystals and their biological activities.
Bioprocess Biosyst Eng
; 42(3): 401-414, 2019 Mar.
Article
en En
| MEDLINE
| ID: mdl-30448964
ABSTRACT
The biosynthesis and biological activity of colloidal Ag2O nanocrystals have not been well studied, although they have potential applications in many fields. For the first time, we developed a reducing agent free, cost-effective technique for Ag2O biosynthesis using Xanthomonas sp. P5. The optimal conditions for Ag2O synthesis were 50 °C, pH 8, and 2.5 mM AgNO3. Using these conditions the yield of Ag2O obtained at 10 h was about five times higher than that obtained at 12 h under unoptimized conditions. Ag2O was characterized by FESEM-EDS, TEM, dynamic light scattering, XRD, and UV-Visible spectroscopy. Indoleacetic acid produced by the strain P2 was involved in the synthesis of Ag2O. Ag2O exhibited a broad antimicrobial spectrum against several human pathogens. Furthermore, Ag2O exhibited 1,1-diphenyl-2-picrylhydrazyl (IC50 = 25.1 µg/ml) and 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonate (IC50 = 16.8 µg/ml) radical scavenging activities, and inhibited collagenase (IC50 = 27.9 mg/ml). Cytotoxicity of Ag2O was tested in fibroblast cells and found to be non-toxic, demonstrating biocompatibility.
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Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Óxidos
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Ensayo de Materiales
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Compuestos de Plata
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Nanopartículas
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Fibroblastos
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Ácidos Indolacéticos
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Antiinfecciosos
Límite:
Animals
Idioma:
En
Año:
2019
Tipo del documento:
Article